- A-Z
- Endocytobiosis and ...
- Volume 30
- Isolation of multi-...
- Kurztitel
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Thylakoid protein complexes
- Autor(in)
- Erschienen
- 19. März 2019
- Referenz
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2019ECR0227_Herrmann R etal
- Seitenbereich
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001-006
- Schlagwort(e)
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thylakoid membranes, photosystem I, photosystem II, cytochrome b6/f complex, CFoF1 ATP synthase
- Zusammenfsg.
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Thylakoids are biomembranes capable of converting inorganic substrates into the ubiquitously utilizable high-energy chemical metabolites ATP and NADPH using the energy of solar light. The photosynthetic membranes are highly dynamic structures consisting of almost equal parts of proteins and electrically isolating lipids in complex arrangements. In this article we describe protocols for the isolation of the four major photosynthetic membrane complexes: the photosystem I and II assemblies, the cytochrome b6/f complex and the CFoF1 ATP synthase. The protocols were originally developed for spinach. They work also with other plant species such as Arabidopsis thaliana, Nicotiana species, Pisum sativum and Hordeum vulgare, but may require some modification in specific cases.