PhostagTM-gel retardation and in situ thylakoid kinase assay for determination of chloroplast protein phosphorylation targets
- Autor(in)
- Seitenbereich
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062-070
- Schlagwort(e)
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chloroplast phosphorylation network, thylakoid kinases.
kinase protein interaction, gel retardation,
posttranslational regulation of photosynthesis
- Zusammenfsg.
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The chloroplast phosphorylation network is important for posttranslational regulation of photosynthetic complexes, gene expression and metabolic pathways. In mass-spectrometric analyses a lot of putative phosphorylation targets have been found but these data need to be confirmed and brought into a physiological context. Here, we present a current protocol to quantify the phosphorylation state of thylakoid proteins and an in situ method to verify putative substrates for thylakoid associated kinases.