The purpose of this study was to isolate psychrophilic fungal strains from the new geographical region Baramullah (Jammu and Kashmir) as a source of cold-active pectinolytic activities of oenological grade. Out of six cold-active fungi, BPF-5 showing fast growth at 4°C and pectinolytic activity on plate was selected and preliminarily characterized. The three pectinolytic enzyme activities i.e., pectin esterase (PE), exo-galacturanase (Exogal) and endo-galacaturanase (Endogal) were isolated and assayed under near oenological conditions (temperatures 12°C and 28°C; pH 3.5). The highest specific activities for PE, exogal and endogal were found to be 472, 31.4 and 64.8 U/mg, respectively. The percentage activity of PE, Exogal and Endogal at pH 3.5 at 12°C were 40%, 60% and 39% and at 28°C were 45%, 60% and 42%, respectively. These pectinolytic enzymes could be of immense importance in wine production and juice industries.