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- Endocytobiosis and ...
- Volume 22 (2012)
- Isolation of Thecam...
- Autor(in)
- Seitenbereich
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037-042
- Schlagwort(e)
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endoparasite, nucleus, Thecamoeba, spore, fine structure, Microsporidia, VLP
- Zusammenfsg.
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Samples of the bark from a Sycamore tree at Andernach were transferred to NN-agar plates. From a mixed population of various free-living amoebae (FLA), few trophozoites of Thecamoeba terricola obviously harbouring intranuclear parasites were isolated. Since all of the original host amoebae were prone to death by massive infections, spores were successfully transferred to a laboratory strain of T. quadrilineata which served as a substitute host of the parasites called “KTt-1” and enabled all following studies. Electron microscopical studies confirmed the exclusive location of the parasites within the nucleus. After uptake by phagocytosis the spores get into the nucleus where they grow and develop over young stages within the endosome into large parasites occupying the whole interior of the nucleus while stretching the nuclear membrane. At this stage they differentiate into round or oval spores of 1.5 – 1.8 µm in diameter. The spores were enveloped by a double wall and contained in addition to the nucleus, a polar filament with an anchoring disc characteristic of microsporidia. Moderately large dark bodies with unknown function could be observed in numerous spores as well. In a single spore an expanded dumbbell-like structure contained numerous small polygonal corpuscles reminiscent of viruses (VLP). Within the cytoplasm of the same host amoeba, rod-shaped Gram-negative bacteria could be observed as additional endosymbionts.
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