- A-Z
- Endocytobiosis and ...
- Volume 21 (2011)
- GFP-based in vivo p...
GFP-based in vivo protein topology determination in plant protoplasts
- Autor(in)
- Seitenbereich
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089-097
- Schlagwort(e)
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protein import, protein topology, protoplast isolation, self-assembly GFP
- Zusammenfsg.
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Since its initial description in 1961 the green fluorescent protein (GFP) has become an indispensable molecular tool in modern cell biology. Genetically fused to a protein of interest, it allows the study of the spatiotemporal expression of the protein within a living cell or tissue. In recent years GFP and its mutants have undergone a multitude of modifications utilizing the protein for a broad spectrum of specific applications such as protein (co-)localization, protein-protein interaction, protein folding, protein transport, protein dynamics and protein turn over. Here we report about GFP as a tool to assess the topology of membrane proteins in vivo.
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