- A-Z
- Endocytobiosis and ...
- Volume 21 (2011)
- An optimized method...
- Author
- size
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034-042
- keyword(s)
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mitochondria, mRNA analysis
- abstract
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Transcriptome analyses enhance our understanding of RNA-based regulatory mechanisms in prokaryotic organisms as well within the nuclear-cytosolic compartments of eukaryotic cells. Higher plant organelles employ complex post-transcriptional regulatory mechanisms, which are studied in a comprehensive fashion by different RNA-methodologies. Northern blot hybridization provides with important data to the "steady-state" level of accumulation of a given transcript in the sample, and thus remains as a standard method for detection and quantitation of RNA levels, despite the advent of other powerful techniques. Here, we provide a modified method for northern blotting which has been optimized for the analysis of plant mitochondrial RNAs.