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143 - 150
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Epifluorescence microscopic observation after DNA specific fluorochrome, DAPI staining of saccoderm desmid, Netrium digitus showed that the number of cytoplasmic organelle nucleoids per semi-cell was about 200 in maximum, the mean value was 120, and most of which are considered to belong to a chloroplast. Some of the nucleoids were rodlike in shape, but they were mostly fibrous and formed networks throughout the chloroplasts. Autoradiographic investigations showed that tritiated thymine was not incorporated into the nucleus, but incorporated into the chloroplasts. After the chase periods, tritiated chloroplast nucleoid DNA were transferred at random to the daughter cells through cell divisions. The transmission mode of the chloroplast nucleoids is also discussed.
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