- Autor(in)
- Seitenbereich
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075 - 082
- Zusammenfsg.
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Cultured cells of the symbiotic dinoflagellate Symbiodinium kawagutii Trench et Blank, derived from the Hawaiian scleractinian coral Montipora verrucosa (Lamarck) Quoy et Gaimard, were analyzed for intracellular localization of ribulose-1.5-bisphosphate carboxylase-oxygenase (RuBisCO EC 4.1.1.39) by the technique of immuno electron microscopy, using gold-conjugated goat anti-rabbit labelling of rabbit-raised antibodies against the holoenzyme and the separated large and small subunits of RuBisCO from Nicotiana sylvestris Spegazzini et Comes. Polyhedral inclusion bodies previously described as being located within the chloroplast were clearly labelled and are regarded as carboxysomes. Carboxysomes are commonly present in autotrophic prokaryotes but are atypical of plastids of eukaryotic plants. Statistically significant labelling was also observed over the chloroplast and pyrenoid. Insignificant labelling was found associated with other organelles and the cytoplasm, and is probably the result of non-specific binding.