- Autor(in)
- Seitenbereich
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037 - 044
- Zusammenfsg.
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The complete nucleotide sequence of a symbiotic bacterial gene coding for a protein which appears to be required for the survival of the host amoebae was determined from a genomic clone isolated from a library of recombinants in bacteriophage lambda-ZAP. The clone contained a DNA segment with an open reading frame of 759 nucleotides, coding for 253 amino acids with a deduced molecular weight of 29,431. The bacterial gene appeared to be a novel gene, not sharing an overall sequence similarity with any known genes. However, it contained a few strings of nucleotides similar to those of yeast GCN4 and yeast-mitochondrial cytochrome b short genes. Also, there were five leucine residues spaced regularly somewhat like in the leucine zipper of the products of GCN4, Fos and Jun genes, suggesting that the protein may be a DNA-binding protein.