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- Volume 16
- Single Cell PCR amp...
Single Cell PCR amplification of genes from the rumen ciliate Entodinium caudatum
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12-18
- Zusammenfsg.
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A method for performing PCR on single cells from the rumen ciliate Entodinium caudatum is described. The method involves incubation in a Chelex 100 solution for the initial DNA extraction. Performing this type of extraction was found essential, rather than relying on cell lysis by heating alone. This is the first reported application of single cell PCR on ciliate genomic DNA for a gene, which encodes a protein.
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