In vitro evolution of translational cis-regulatory elements
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385-389a
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It is an intriguing topic of endocytobiology how endosymbiont-derived nuclear genes acquire cis-regulatory elements which adjust their expression to the eukaryotic cellular signals. To approach this subject, we developed a new experimental method by which we can observe the evolutionary process of translational regulatory cis-elements of eukaryotic mRNAs. This method consists of the insertion of random nucleotide motifs into the UTRs of given mRNAs, followed by the repeated selections of highly or poorly translatable mRNAs owning to the inserted motifs. Using this method, we show that the 5' cap structure has a great influence on how translational regulatory motifs evolved on the mRNA molecule.
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