- A-Z
- Endocytobiosis and ...
- Volume 28
- Unanticipated T7 RN...
Unanticipated T7 RNA polymerase activity using annealed oligonucleotides as transcription template
- Kurztitel
-
Unanticipated T7 RNA polymerase promoter activity
- Autor(in)
- Seitenbereich
-
033-037
- Schlagwort(e)
-
T7 RNA polymerase, in vitro transcription, RNA synthesis, T7 promoter
- Zusammenfsg.
-
The bacteriophage T7 RNA polymerase (RNAP) is an important tool used in molecular biology to efficiently synthesize specific RNA sequences for diverse in vitro applications. Its activity depends on a DNA template that must contain a specific double-stranded short promoter element. During ongoing work, we noticed an unanticipated activity of the T7 RNAP, which was able to generate RNAs even in the absence of a known T7 promoter motif. Here, we describe the observed artificial activity of the T7 RNAP and attempt to uncover the underlying mechanism.