On the Occasion of the "International Conference on the Molecular Biology and Biotechnology of Ciliates and Anaerobic Protozoa in connection with the 22nd Annual meeting of the German Society for Protozoology" (March 4-8, 2003, Nijmegen, The Netherlands)
In this article we summarize results obtained in the last years studying the organization of the end-replication machinery in spirotrichous ciliates. We could show that the p43-telomerase complex is bound to the nuclear matrix in vivo. After inhibition of p43 expression telomerase is released from this structure, strongly suggesting that p43 is involved in anchoring telomerase in the nucleus as it has been proposed earlier. This was the first in vivo demonstration of the biological function of this telomerase associated component involved in telomere replication and allowed us to introduce a model for the organization of the end-replication machinery in eukaryotic cells. Additionally, this was the first time that RNAi was used to study gene function in spirotrichous ciliates.
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