A symbiont gene coding for a protein required for the host amoeba - cloning and expression in phage-transformed Issue 2 Volume 5 Endocytobiosis and Cell Research A genomic expression library was prepared from DNA of symbiotic bacteria in the xD strain of Amoeba proteus, from which an 8-kb DNA fragment containing the gene coding for the symbiont-synthesized 29-kD protein was isolated and cloned. The inserts were subcloned in an expression vector and transferred to E. coli cells in which the gene was expressed at a high level, as tested by analyzing the bacterial total proteins by gel electrophoresis and immunoblotting. The transformed bacteria exported the fusion protein into the culture medium. 215 - 224 1988 1984 jportal_jpjournal_00000006 2007-08-02T10:15:07.461Z 2010-12-16T12:05:17.022Z