The presence of a spectrin-like protein on symbiosome membranes of symbiont bearing Amoeba proteus as studied with monoclonal antibodies
- Autor(in)
- Seitenbereich
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099 - 108
- Zusammenfsg.
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Monoclonal antibodies were prepared against a high-molecularweight (225/220 kD) protein first found on the symbiosome membranes of xD strain of Amoeba proteus and they were used to determine some of the characteristics and distribution of the protein. A total of 34 monoclonal antibodies recognizing different epitopes were obtained, of which seven stained the antigen by indirect immunofluorescence. The membrane protein was resolved into two bands of 225 kD and 220 kD in 5% SDS-polyacrylamide gels and several monoclonal antibodies cross-reacted with human erythrocyte spectrin when checked by immunoblotting and indirect immunofluorescence staining, indicating that the 225/220-kD protein shares antigenic determinants with spectrin.