- Autor(in)
- Seitenbereich
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057 - 074
- Zusammenfsg.
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The presence of lectin in root hairs of Pisum sativum, cv. Rosa Krone seedlings was demonstrated and its concentration estimated. In addition, the distribution of lectins among root tissue and root hair cells was studied with seedlings grown under different nutritional regimes. Nitrate concentrations in the growth medium inhibiting nodulation, and also the absence of added nitrate did not significantly change the concentrations of lectins in root hairs. No enrichment of lectins in isolated root hairs over their levels in whole roots was detected. The attachment of radioactively labeled cells of Rhizobium leguminosarum PF2 to isolated root hair cells of Pisum sativum cv. Kleine Rheinländerin was studied. Bacterial attachment was not decreased by preincubation of isolated root hairs with antibodies against Pisum sativum seed lectin. The binding to root hairs of cells grown in the absence of exudate was even enhanced by anti-lectin antibodies. Pretreatment of the bacteria with Pisum root exudates reversed this effect.