Endocytobiosis and Cell R... \ Volume 11 \ Issue 2-3 \ Dose-dependent functional...
Dose-dependent functional and morphological alteration of granulocytes after single dose exposure
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Seitenbereich225 - 232

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Zusammenfsg.Background: Granulocytes are considered to be highly radioresistant with functional and morphological changes occurring only after about 100 Gray. Methods: Fresh blood samples of healthy persons were examined after heparinisation. Unirradiated controls and irradiated samples (30 Gray, 60 Gray) were analyzed under light microscopy at 37 degrees C. Changes over time were documented by means of a video camera. Evaluation criteria of morphological changes were: length and overall dimension of granulocytes, development of pseudopodia and fluctuation of granula. Evaluation criteria of functional changes were: maximal migration velocity and intercellular adhesion capacity. Results: Exposure of 60 Gray decreased maximal cell length to 80% of normal cell length, the fluctuation of granula was reduced by one scale on a subjective observer based scale. The maximal migration velocity was reduced by > 20%; moreover, the number of cell contacts decreased during a period of about 15 minutes. In contrast to the observed effects at 60 Gray, no remarkable changes could be demonstrated after a dose of 30 Gray. Conclusion: After a dose of 60 Gray the cellular dynamic functions appear to be reduced, the morphological variability is decreased. The principal functions remain unchanged.
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Statische URLhttp://zs.thulb.uni-jena.de/receive/jportal_jparticle_00059818